Shiitake Spawn Production

Slants are held to maintain young cell lines

When a culture is needed, cells are brought back to life by inoculating petri plates contain a variety of agar media formulations.

Three strains ready to move to rye grain.

Scalpel being flame sterilized.

Scalpel being cooled.

Mycelium being cut.

Mycelium being transferred to grain.

After transfer plate will be sealed or used again for more transfers.

The wedge in the grain .

Three weeks later jar is colonized.

Close up of shiitake mycelium growing on grain.

The mother spawn is used to make more grain spawn which is then used to inoculate sawdust to fruit the mushrooms.

Shiitake Substrate Production

Sawdust is loaded into container.

The sawdust is then added to a mixing vessel.

Wheat bran is also added to mixing vessel.

The dry ingredients are mixed by hand.

Water is added to bring the moisture content of the mix to optimal level.

The bags with gas exchange filters.

Nine bags weighing 4.5 pounds each are filled every day.

They are loaded into the sterilizer and steam sterilized for 90 minutes.

Spawn made from our highest quality stocks.

Spawns appearance after shaking and breaking apart the colonized rye grain.

Opening a sawdust bag to be inoculated in front of a laminar flow hood.

Bags are sealed and placed on shelves to incubate.

After 4 days the mycelium begins to grow into the wood substrate.

After 3 weeks mycelium is colonizing rapidly and binding the substrate tightly together.

Bags incubating on shelves.

The mycelium begins to buckle and form a brown coat after wood is covered with the white mycelium.

After approximately two months bags are fully colonized and mushrooms fruit body induction is started.

The bags are removed and the new born block is rinsed and set placed in fruiting conditions with high humidity and lower temperatures.

Close up block in mushroom grow area just before primordia form.

Small mushroom emerge through the brown coat.

Bags incubating on shelves.